LOCUS       40924_44829       10374 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-Hyg DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 10374)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(320..1342)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1410..2087)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    2278..2299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2314..2344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2352..2368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2376..2392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2414..2666)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2713..2729)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(2730..3316)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(2730..3269)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(3270..3316)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(3317..3851)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(3878..3894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4097..4121
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4116..4141
                     /label=right border T-DNA repeat
                     /note="right border T-DNA repeat"
     CDS             5421..6047
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6484..7548
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7617..7811
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8155..8295
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8481..9069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9159..9950)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
      301 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
      361 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
      421 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
      481 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
      541 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
      601 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
      661 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
      721 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
      781 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
      841 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
      901 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
      961 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
     1021 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     1081 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     1141 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     1201 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     1261 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     1321 cggtgagttc aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga
     1381 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     1441 ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     1501 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     1561 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     1621 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     1681 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     1741 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     1801 tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag
     1861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     1921 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     1981 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     2041 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     2101 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     2161 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2221 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2281 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2341 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2401 cgaattcgag ctcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     2461 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     2521 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     2581 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     2641 ctttattgcc aaatgtttga acgatcgatg gcgcgcctag gtaccggatc cactagtagg
     2701 ccttctaggg gggatccact agttctagac tgtaatcaca tcaacaacag ataaatcaga
     2761 atccaacaca cgaaattgaa acaagtgaaa accctaatta acgcttggat caaacagatt
     2821 aagagtggat ctaacatcaa cagataaatc agaatccata acaagaaatt gaaacaagtg
     2881 aaaaccctaa ttgaagcttg gatcaaacag attaagagtg gatctaacaa taatttccaa
     2941 tttgaataaa ttatttgaca taattccata acggatctga agaattcaat tggaaaccct
     3001 aattaatctg caattgctaa accgaaatcg attcctaaca aaactgaacc acaattatca
     3061 tagatctaca tcgcaagcca tcgaaaacat tgataaaaca agatctgaga ttcaagctgt
     3121 aaaattcaat ttcaaaccga tctcaatttt ggaaaatcac aaaaaattcc tagatctcat
     3181 gcttcgaaac aagaaaacta cctatgattg atcggaaaca aaactcaacg aaaggattac
     3241 agagaaagaa gagagaaatt ggatcatacc ttgaaagtag agagaagcga tatgaacaag
     3301 aattgttagg gttgggaact gaattgtgaa gaagctaagg ggattggagg taatttatag
     3361 caaaagcggt gaaattatta accagtcaaa ttgtgacacg tgtccttatg acgcgtcact
     3421 agttgcgtgt ggtttgggat aggatacgtt gtgtggttga gttcttcttg gaaacggtag
     3481 ttgcaaattg caagttgttg gcggtttgtt aatttcacgg agttaaacgc atctcgccgc
     3541 cttttgagtt tggattgata aatcgttaca acggcgttac tttgctgggc cccgtttttt
     3601 ttgaattggg ccaccgcacc agtgcaagtt gggggttcct tggttttcca gaatgggctc
     3661 cactctctat gattgattgg aggaggccgg ctgctcagaa attttataat aagttgttat
     3721 atacttctcc ccttaaaata agtgattttt gtctatccat agttgcaata agttgtacta
     3781 acaacttatc taaattactt tatcaataat acaagtaatt caattaatta tttcctcaaa
     3841 atcctctctc catcgaattc ctgcagccca gcttggcact ggccgtcgtt ttacaacgtc
     3901 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3961 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4021 tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt
     4081 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     4141 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     4201 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     4261 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     4321 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     4381 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     4441 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     4501 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     4561 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     4621 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     4681 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     4741 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     4801 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     4861 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     4921 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     4981 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     5041 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     5101 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     5161 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     5221 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     5281 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     5341 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     5401 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     5461 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     5521 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     5581 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     5641 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     5701 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     5761 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     5821 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     5881 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     5941 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     6001 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     6061 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     6121 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     6181 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     6241 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     6301 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     6361 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     6421 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     6481 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     6541 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     6601 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     6661 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     6721 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     6781 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     6841 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     6901 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     6961 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     7021 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     7081 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     7141 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     7201 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     7261 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     7321 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     7381 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     7441 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     7501 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     7561 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     7621 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     7681 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     7741 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     7801 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     7861 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     7921 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     7981 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     8041 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8101 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     8161 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     8221 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9121 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
     9181 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     9241 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     9301 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     9361 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     9421 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
     9481 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     9541 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     9601 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     9661 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     9721 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     9781 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     9841 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
     9901 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
     9961 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    10021 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    10081 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    10141 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    10201 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    10261 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    10321 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctgg
//
